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Effects of N-acetylcysteine treatment on frontal glutamate and GABA levels and associations with drinking quantity in people with co-occurring posttraumatic stress disorder and alcohol use disorder

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Protein preparation and immunoblot analysis Whole rosettes from 4-week-old plants, which had been grown for 2 weeks under normal light followed by 2-week growth under high light, were collected and ground in liquid nitrogen, after which ~60 mg of the powder was homogenized with 500 L Extraction Buffer [150 mM Tris-HCl (pH 6.8), 7.5% -mercaptoethanol, 3% sodium dodecyl sulfate, and half a cOmplete mini protease inhibitor tablet (Roche)]

Lastly, metformin treatment did not significantly alter the MDA increase and SOD decrease induced by MIA injection (OA model) in Nrf2 KO mice (Fig