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After 2 h, the Agrobacterium culture (OD 600 = 1.0) harboring pBI121-GFP was injected into leaves, and RNA silencing was determined by observing the GFP fluorescence intensity in the infiltrated and newly emerging leaves under long-wavelength ultraviolet light for 7 days

Toxins and nicotine have been measured in that aerosol, such as formaldehyde, acetaldehyde, acetic acid and other toxins emitted into the air, though at lower levels compared to conventional cigarette emissions