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A healthy, unstressed cell maintains a vast majority of its glutathione pool in the reduced state, typically sustaining a GSH-to-GSSG ratio of greater than 10:1, and often up to 100:1 in the cytosol

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6 and 7, Table 1)

Immunofluorescence microscopy was performed using the confocal microscopes LSM 510 Meta and LSM 780 (Carl Zeiss, Jena, Germany) equipped with the objective lenses Plan-Apochromat 10/0.3, Plan-Neofluar 20/0.5, Plan-Neofluar 40/1.3 oil differential interference contrast, Plan-Apochromat 63/1.4 oil differential interference contrast (Carl Zeiss)