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Isolation of PBMC PBMC were immediately isolated from fresh whole blood by density gradient centrifugation according to the method of Boyun 50 using equal volume of blood and Ficoll (Histo-paque 1077)

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His innovative designcomprising a battery-powered heating element, an atomizer, and a nicotine solutionpaved the way for the evolution of vaping technology and the commercial production of e-cigarettes