They used AlphaFold2 to predict the structure of the original sensor, removed the unstructured linker computationally, and reoriented Grx1 to align the GSSG binding site with the reactive cysteines of roGFP2
R471C has been shown to localise to the membrane and has ~15% activity of wildtype OCTN2 in CHO cells, which is consistent with the observed reduction in currents for R471A, meanwhile Y358N in CHO cells has ~8% activity, though localisation data for Y358N is unavailable 11,50
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In an email to Stateline, Werner said coverage of GLP1s for weight loss would be reconsidered if Medicaid is fully funded. Some states are trying to maintain at least some coverage of the expensive drugs by tightening the eligibility requirements for a prescription, according to Colleen Becker, a project manager at the National Conference of State Legislatures, a policy research group