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The diets used in this study comprised three free-choice diets in which rats could unrestrictedly eat any of the diet components: (1) Free-choice high-fat high-sugar diet (fcHFHS): a dish of saturated fat [Beef tallow (Ossewit/Blanc de Boeuf), Vandemoortele, Belgium], a bottle of 30% sugar water (1.0 M sugar mixed from commercial grade sugar and tap water), normal standard chow [special diet service (SDS), England] and normal tap water

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