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Aliquots (30 l) were taken at various timepoints (0, 1, 4, 8, 24 h) and added to vials which had been pre-filled with 20 l of 0.05% TFA in water, and gently mixed

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That paper, along with trial data from STEP, SURMOUNT, and SURPASS, forms basis for approximate conversion ranges used in clinical practice today

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