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Methods C2C12 cells were differentiated into myotubes by growing them in DMEM for 24 h (hrs) and then changing the media to DMEM supplemented with 2% horse serum

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Carbohydrate, we are basically lowering it until we reach the highest point...or pardon me, the lowest point that we can tolerate where we can maintain, and again, this is quick and dirty but it's the lowest possible fasting insulin

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